《植物生理学报》 2017, 53(3): 505-512

非洲菊花瓣瞬时表达和病毒诱导的基因沉默(VIGS)系统的建立

唐宜¹, 李凌飞^1,2,*, 王小菁¹

¹华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631; ²深圳市中国科学院仙湖植物园, 深圳市南亚热带植物多样性重点实验室, 广东深圳518004

通信作者：李凌飞；E-mail: lingfei_li@m.scnu.edu.cn

摘　要：

非洲菊(Gerbera hybrida)是重要的园艺观赏花卉, 也是研究复杂花序进化与发育的模式植物。花瓣的生长对园艺观赏花卉品质的形成具有重要贡献, 阐明花瓣生长的分子机理将有助于增加对植物器官形态建成的认识, 并为花卉品质的改良提供理论依据。为了在非洲菊中更好地开展基因功能研究, 建立有效的转化系统十分必要。本研究以非洲菊舌状花花瓣为材料, 通过农杆菌真空渗透法, 分别以β-葡萄糖苷酸酶基因GUS和花色素苷合成关键转录因子基因GMYB10为报告基因, 成功建立了花瓣瞬时表达及病毒诱导的基因沉默(virus-induced gene silence, VIGS)系统。该系统将为今后快速有效地开展非洲菊花瓣发育相关基因功能研究奠定技术基础。

关键词：非洲菊; 花瓣; 瞬时基因表达系统; 病毒诱导的基因沉默

收稿：2017-01-04   修定：2017-02-16

资助：国家自然科学基金(31372099和31601784)、教育部高等学校博士学科点专项科研基金资助课题(20104407110005)和广东省自然科学基金重点项目(9251063101000002)。

Establishment of transient gene expression and virus-induced gene silencing (VIGS) system in Gerbera hybrida petals

TANG Yi¹, LI Ling-Fei^1,2,*, WANG Xiao-Jing¹

¹School of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou 510631, China; ²Key Laboratory of Southern Subtropical Plant Diversity, Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen, Guangdong 518004, China

Corresponding author: LI Ling-Fei; E-mail: lingfei_li@m.scnu.edu.cn

Abstract:

Gerbera hybrida is an important ornamental flower, and also an ideal model system for studying development and evolution mechanism of complex inflorescences. Petal growth is pivotal for ornamental flowers morphogenesis. Clarifying the genetic networks of petal morphogenesis will contribute to understand the organogenesis as well as provide new theoretical basis for ornamental plant breeding. Thus, it is necessary to establish an effective transformation system for in-depth identification of gene function in G. hybrida. In this study, β-glucuronidase (GUS) and GMYB10 (key gene for anthocyanin biosynthesis in Gerbera petal) were selected as reporter genes to establish transient gene expression and virus-induced gene silencing (VIGS) system in Gerbera ray petals. Using Agrobacterium-mediated vacuum infiltration method, high-efficiency transformation systems had been successfully established. This study will provide an effective tool for gene function analysis in G. hybrida petals.

Key words: Gerbera hybrida; petal; transient gene expression system; virus-induced gene silencing